hs 4800pro hybridization station  (Tecan Systems)

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Comparative Genomic Hybridization: DNA labeling, hybridization and detection

doi: 10.1007/978-1-59745-538-1_17

Figure Lengend Snippet: Array-CGH program for Hs. PRO stations (Tecan, Inc)

Article Snippet: Hs.400PRO/4800PRO automated slide processing station with 51×20mm hybridization chambers (Tecan, Inc).

Techniques: Injection, Hybridization, Viscosity

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Comparative Genomic Hybridization: DNA labeling, hybridization and detection

doi: 10.1007/978-1-59745-538-1_17

Figure Lengend Snippet: (A) Merged images displaying signal intensities after comparative genomic hybridization of one test female DNA (labeled with Cy3, in green) versus one reference male DNA (labeled with Cy5, in red) on a microarray covering the entire human genome with ~30,000 large-insert clones (16). The microarray (left panel) is divided in 8×4 blocks, each of them being composed of 31×31 spots (right panel). As both DNA samples are from healthy anonymous individuals, most spots (clones) show no copy number changes and appear in yellow (green = red = 2 DNA copies). However, on each block (right panel), a few clones appear in green or in red: most of them are clones mapped on chromosome X (green = 2, red = 1) or on chromosome Y (green = 0, red = 1). (B) Whole genome array-CGH profile derived from the images merged in (A). For each spot, the log2ratio value is calculated, normalized by the median of all log2ratio values and plotted against the genome position of the corresponding clone. The profile shows log2ratio values that are close to zero on autosomes, increased (up to +1) on chromosome X and decreased (down to less than −3) on chromosome Y. Some local log2ratio variations indicate the presence of small copy number changes between the 2 individuals (examples indicated by red arrows).

Article Snippet: Hs.400PRO/4800PRO automated slide processing station with 51×20mm hybridization chambers (Tecan, Inc).

Techniques: Hybridization, Labeling, Microarray, Clone Assay, Blocking Assay, Derivative Assay